- Screening of both natural and synthetic entities and understanding the mode of action of the lead molecules.
- Target-based biochemical screens
- Cell-based phenotypic screens
- Gene expression based screening
- RNAi screening combined with small molecules
- Assay development
- Analysis of cellular bioenergetics
- Neurotransmitter analysis
- Stem Cells for regenerative medicines
- Cancer Stem Cells for advance anti-cancer screening
- In vivo models of liver fibrosis and cirrhosis
- In vivo models of few neuropsychiatric and neurological disorders
- To decipher the pathway-specific disease mechanisms using the lead molecules in the areas of cancer, cardiovascular, metabolic and CNS disorders.
- Protein crystallography
- Recombinant expression and protein engineering for medical and biotechnology applications
- Implementation of bioinformatics, structural biology and molecular modeling strategies to understand the structure-function relationship of the lead molecules.
- Identification of molecular targets / potential biomarkers and their validation.
- Proof-of-concept using appropriate animal models
The major activities of the department are as follows:
- Primary screening of molecules, both natural and synthetic origin employing various screening platforms such as, target, cell, RNAi, gene expression and proteomics based methods for diseases such as cancer, diabetes, cardiovascular, CNS disorders and tuberculosis.
- Application of structural biology and protein engineering tools to understand the interaction between small molecule and protein targets. Structure-activity relationship studies for refinement of lead molecules to improve efficacy.
- Directed and basic research in the aforementioned disease areas is also actively pursued to understand the disease mechanisms and, identification of new druggable targets and biomarkers.
- Cellular and molecular mechanisms mediating neurodegeneration, cardiovascular diseases, cancer, oxidative stress and inflammation.
- Stem cells and regenerative medicine, cell signaling in angiogenesis, liver diseases.
- Application of RNAi, transcriptomics, proteomics, lipidomics structural biology, protein engineering, biophysics, bioinformatics and molecular modeling, medicinal chemistry, protein crystallography tools.
- Exploration of signaling chemicals, biocatalyst enzymes, bioactive compounds, bioethanol, biosurfactants and microbial transformation of fatty acids.